SubtiBank SubtiBank
Version comparison:

2017-06-23 17:21:272017-06-08 10:44:55

The protein

Modification

transient phosphorylation by [[protein|PtsI|Enzyme I]]]] of the PTS on His-15

regulatory phosphorylation on Ser-46 by [[protein|HprK]] [Pubmed|2507315]

an extensive study on ''in vivo'' HPr phosphorylation can be found in Singh et al. (2008) [PubMed|18757537]

weak phosphorylation on Ser-12 [Pubmed|17218307]

''in vitro'' phosphorylated by [[protein|PrkC]] on Ser-12 [Pubmed|20389117]

transient phosphorylation by [[protein|PtsI|Enzyme I]]]] of the PTS on His-15

regulatory phosphorylation on Ser-46 by [[protein|HprK]] [Pubmed|2507315]

an extensive study on ''in vivo'' HPr phosphorylation can be found in Singh et al. (2008) [http://www.ncbi.nlm.nih.gov/sites/entrez/18757537 PubMed]

weak phosphorylation on Ser-12 [Pubmed|17218307]

''in vitro'' phosphorylated by [[protein|PrkC]] on Ser-12 [Pubmed|20389117]

The protein

Structure

[PDB|2HID] (NMR) [Pubmed|9336834]

[PDB|1KKM] (complex of ''L. casei'' [[protein|HprK]] with ''B. subtilis'' HPr-Ser-P)

[PDB|1KKL] (complex of ''Lactobacillus casei'' [[protein|HprK]] with ''B. subtilis'' HPr)

[PDB|3OQM] (complex of ''B. subtilis'' CcpA with P-Ser-[[protein|PtsH|HPr]] and the [[gene|ackA]] operator site)

[PDB|3OQN] (complex of ''B. subtilis'' CcpA with P-Ser-[[protein|PtsH|HPr]] and the [[gene|gntR]] operator site)

[PDB|3OQO] (complex of ''B. subtilis'' CcpA with P-Ser-[[protein|PtsH|HPr]] and a optimal synthetic operator site)

[PDB|2HID] (NMR) [Pubmed|9336834]

[PDB|1KKM] (complex of ''L. casei'' [[protein|HprK]] with ''B. subtilis'' HPr-Ser-P)

[PDB|1KKL] (complex of ''Lactobacillus casei'' [[protein|HprK]] with ''B. subtilis'' HPr)

[PDB|3OQM] (complex of ''B. subtilis'' CcpA with P-Ser-[[protein|PtsH|HPr]]]] and the ''[[gene|ackA]]'' operator site)

[PDB|3OQN] (complex of ''B. subtilis'' CcpA with P-Ser-[[protein|PtsH|HPr]]]] and the ''[[gene|gntR]]'' operator site)

[PDB|3OQO] (complex of ''B. subtilis'' CcpA with P-Ser-[[protein|PtsH|HPr]]]] and a optimal synthetic operator site)

Biological materials

Mutant

available in [SW|Jörg Stülke]'s lab:

MZ303 (cat)

GP507 ptsH1 (S46A)

GP506 (ptsH-H15A)

GP778 (replacement of [[gene|glcT]] and the [[gene|ptsG]]-[[gene|ptsH]]-[[gene|ptsI]] operon by a spc cassette), [Pubmed|22722928]

available in [SW|Jörg Stülke]'s lab:

MZ303 (cat)

GP507 ptsH1 (S46A)

GP506 (ptsH-H15A)

GP778 (replacement of ''[[gene|glcT]]'' and the ''[[gene|ptsG]]-[[gene|ptsH]]-[[gene|ptsI]]'' operon by a spc cassette), [Pubmed|22722928]