2017-06-23 17:21:272017-06-08 10:44:55
The protein
Modification
transient phosphorylation by [[protein|PtsI|Enzyme I]]]] of the PTS on His-15
regulatory phosphorylation on Ser-46 by [[protein|HprK]] [Pubmed|2507315]
an extensive study on ''in vivo'' HPr phosphorylation can be found in Singh et al. (2008) [PubMed|18757537]
weak phosphorylation on Ser-12 [Pubmed|17218307]
''in vitro'' phosphorylated by [[protein|PrkC]] on Ser-12 [Pubmed|20389117]
transient phosphorylation by [[protein|PtsI|Enzyme I]]]] of the PTS on His-15
regulatory phosphorylation on Ser-46 by [[protein|HprK]] [Pubmed|2507315]
an extensive study on ''in vivo'' HPr phosphorylation can be found in Singh et al. (2008) [http://www.ncbi.nlm.nih.gov/sites/entrez/18757537 PubMed]
weak phosphorylation on Ser-12 [Pubmed|17218307]
''in vitro'' phosphorylated by [[protein|PrkC]] on Ser-12 [Pubmed|20389117]
The protein
Structure
[PDB|2HID] (NMR) [Pubmed|9336834]
[PDB|1KKM] (complex of ''L. casei'' [[protein|HprK]] with ''B. subtilis'' HPr-Ser-P)
[PDB|1KKL] (complex of ''Lactobacillus casei'' [[protein|HprK]] with ''B. subtilis'' HPr)
[PDB|3OQM] (complex of ''B. subtilis'' CcpA with P-Ser-[[protein|PtsH|HPr]] and the [[gene|ackA]] operator site)
[PDB|3OQN] (complex of ''B. subtilis'' CcpA with P-Ser-[[protein|PtsH|HPr]] and the [[gene|gntR]] operator site)
[PDB|3OQO] (complex of ''B. subtilis'' CcpA with P-Ser-[[protein|PtsH|HPr]] and a optimal synthetic operator site)
[PDB|2HID] (NMR) [Pubmed|9336834]
[PDB|1KKM] (complex of ''L. casei'' [[protein|HprK]] with ''B. subtilis'' HPr-Ser-P)
[PDB|1KKL] (complex of ''Lactobacillus casei'' [[protein|HprK]] with ''B. subtilis'' HPr)
[PDB|3OQM] (complex of ''B. subtilis'' CcpA with P-Ser-[[protein|PtsH|HPr]]]] and the ''[[gene|ackA]]'' operator site)
[PDB|3OQN] (complex of ''B. subtilis'' CcpA with P-Ser-[[protein|PtsH|HPr]]]] and the ''[[gene|gntR]]'' operator site)
[PDB|3OQO] (complex of ''B. subtilis'' CcpA with P-Ser-[[protein|PtsH|HPr]]]] and a optimal synthetic operator site)
Biological materials
Mutant
available in [SW|Jörg Stülke]'s lab:
MZ303 (cat)
GP507 ptsH1 (S46A)
GP506 (ptsH-H15A)
GP778 (replacement of [[gene|glcT]] and the [[gene|ptsG]]-[[gene|ptsH]]-[[gene|ptsI]] operon by a spc cassette), [Pubmed|22722928]
available in [SW|Jörg Stülke]'s lab:
MZ303 (cat)
GP507 ptsH1 (S46A)
GP506 (ptsH-H15A)
GP778 (replacement of ''[[gene|glcT]]'' and the ''[[gene|ptsG]]-[[gene|ptsH]]-[[gene|ptsI]]'' operon by a spc cassette), [Pubmed|22722928]